Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/Romanoski_4_2.Romanoski_4_2.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 30285604 30285604
Not Truncated 23775297 23726628
Truncated 6510307 6558976
Too short to map 567326 587996
Average length of truncated sequence 52.96 52.69
  Read 1 Read 2
Unique Alignments 24890399 24537026
Multiple Alignments 3534517 3814813
Failed To Align 1293362 1345769
Paired 20981323 20981323

Filtering

  Di-Tag Count
Valid Pairs 17844589
Invalid Pairs 3136734
Same Circularised 118604
Same Fragment Dangling Ends 288011
Same Fragment Internal 896900
Re-ligation 479387
Contiguous Sequence 29319
Wrong Size 1324513
Total Pairs 20981323

Di-tag length Distribution


De-duplication

Percentage uniques: 94.13

  All Di-Tags Unique Di-Tags
Read Pairs 17844589 16796842
Cis-close (< 10Kbp) 1178905 1107828
Cis-far (> 10Kbp) 13240709 12485131
Trans 3424975 3203883