Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/Romanoski_4.Romanoski_4.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 27266908 27266908
Not Truncated 21326747 21450216
Truncated 5940161 5816692
Too short to map 517984 532088
Average length of truncated sequence 52.93 52.57
  Read 1 Read 2
Unique Alignments 22573663 21588766
Multiple Alignments 3159545 3660095
Failed To Align 1015716 1485959
Paired 18631217 18631217

Filtering

  Di-Tag Count
Valid Pairs 15829703
Invalid Pairs 2801514
Same Circularised 106969
Same Fragment Dangling Ends 259155
Same Fragment Internal 804420
Re-ligation 427962
Contiguous Sequence 25986
Wrong Size 1177022
Total Pairs 18631217

Di-tag length Distribution


De-duplication

Percentage uniques: 94.58

  All Di-Tags Unique Di-Tags
Read Pairs 15829703 14971281
Cis-close (< 10Kbp) 1051008 992026
Cis-far (> 10Kbp) 11736447 11118858
Trans 3042248 2860397