Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/Romanoski_3_2.Romanoski_3_2.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 31376814 31376814
Not Truncated 24379395 24291774
Truncated 6997419 7085040
Too short to map 602484 636999
Average length of truncated sequence 53.08 52.69
  Read 1 Read 2
Unique Alignments 25849540 25386984
Multiple Alignments 3800182 4069471
Failed To Align 1124608 1283360
Paired 21749845 21749845

Filtering

  Di-Tag Count
Valid Pairs 18981822
Invalid Pairs 2768023
Same Circularised 119865
Same Fragment Dangling Ends 242601
Same Fragment Internal 753646
Re-ligation 461262
Contiguous Sequence 29283
Wrong Size 1161366
Total Pairs 21749845

Di-tag length Distribution


De-duplication

Percentage uniques: 93.80

  All Di-Tags Unique Di-Tags
Read Pairs 18981822 17804389
Cis-close (< 10Kbp) 1211908 1134309
Cis-far (> 10Kbp) 14099656 13247028
Trans 3670258 3423052