Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/Romanoski_3.Romanoski_3.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 26307203 26307203
Not Truncated 20342401 20434183
Truncated 5964802 5873020
Too short to map 516729 538285
Average length of truncated sequence 52.98 52.60
  Read 1 Read 2
Unique Alignments 21805857 20788478
Multiple Alignments 3167711 3646892
Failed To Align 816906 1333548
Paired 17951656 17951656

Filtering

  Di-Tag Count
Valid Pairs 15651121
Invalid Pairs 2300535
Same Circularised 100968
Same Fragment Dangling Ends 201191
Same Fragment Internal 630430
Re-ligation 383325
Contiguous Sequence 24400
Wrong Size 960221
Total Pairs 17951656

Di-tag length Distribution


De-duplication

Percentage uniques: 94.61

  All Di-Tags Unique Di-Tags
Read Pairs 15651121 14807041
Cis-close (< 10Kbp) 1003610 947489
Cis-far (> 10Kbp) 11614395 11004132
Trans 3033116 2855420