Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/Romanoski_2_2.Romanoski_2_2.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 35289700 35289700
Not Truncated 27468897 27457006
Truncated 7820803 7832694
Too short to map 722288 742893
Average length of truncated sequence 52.28 52.04
  Read 1 Read 2
Unique Alignments 28655262 28177922
Multiple Alignments 4487119 4783242
Failed To Align 1425031 1585643
Paired 23997573 23997573

Filtering

  Di-Tag Count
Valid Pairs 20342101
Invalid Pairs 3655472
Same Circularised 134726
Same Fragment Dangling Ends 405409
Same Fragment Internal 943574
Re-ligation 592469
Contiguous Sequence 33207
Wrong Size 1546087
Total Pairs 23997573

Di-tag length Distribution


De-duplication

Percentage uniques: 89.95

  All Di-Tags Unique Di-Tags
Read Pairs 20342101 18298636
Cis-close (< 10Kbp) 1326337 1189826
Cis-far (> 10Kbp) 14633526 13202829
Trans 4382238 3905981