Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/Romanoski_2.Romanoski_2.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 30135904 30135904
Not Truncated 23367664 23542313
Truncated 6768240 6593591
Too short to map 627960 637591
Average length of truncated sequence 52.23 51.91
  Read 1 Read 2
Unique Alignments 24639059 23512471
Multiple Alignments 3804272 4341757
Failed To Align 1064613 1644085
Paired 20192717 20192717

Filtering

  Di-Tag Count
Valid Pairs 17102584
Invalid Pairs 3090133
Same Circularised 114709
Same Fragment Dangling Ends 343566
Same Fragment Internal 799487
Re-ligation 501073
Contiguous Sequence 28325
Wrong Size 1302973
Total Pairs 20192717

Di-tag length Distribution


De-duplication

Percentage uniques: 91.05

  All Di-Tags Unique Di-Tags
Read Pairs 17102584 15572649
Cis-close (< 10Kbp) 1116952 1014260
Cis-far (> 10Kbp) 12293167 11225016
Trans 3692465 3333373