Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/Romanoski_1_2.Romanoski_1_2.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 34137523 34137523
Not Truncated 27398200 27413683
Truncated 6739323 6723840
Too short to map 601695 619203
Average length of truncated sequence 52.78 52.50
  Read 1 Read 2
Unique Alignments 27971023 27290782
Multiple Alignments 4041951 4396986
Failed To Align 1522854 1830552
Paired 23274004 23274004

Filtering

  Di-Tag Count
Valid Pairs 18695031
Invalid Pairs 4578973
Same Circularised 123016
Same Fragment Dangling Ends 425695
Same Fragment Internal 1240767
Re-ligation 745153
Contiguous Sequence 40300
Wrong Size 2004042
Total Pairs 23274004

Di-tag length Distribution


De-duplication

Percentage uniques: 87.89

  All Di-Tags Unique Di-Tags
Read Pairs 18695031 16431866
Cis-close (< 10Kbp) 1190497 1044888
Cis-far (> 10Kbp) 13284229 11718238
Trans 4220305 3668740