Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/Romanoski_1.Romanoski_1.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 31444784 31444784
Not Truncated 25203933 25383229
Truncated 6240851 6061555
Too short to map 559345 570704
Average length of truncated sequence 52.74 52.37
  Read 1 Read 2
Unique Alignments 25942675 24559182
Multiple Alignments 3702352 4338711
Failed To Align 1240412 1976187
Paired 21126114 21126114

Filtering

  Di-Tag Count
Valid Pairs 16945089
Invalid Pairs 4181025
Same Circularised 113442
Same Fragment Dangling Ends 387316
Same Fragment Internal 1126923
Re-ligation 679384
Contiguous Sequence 37120
Wrong Size 1836840
Total Pairs 21126114

Di-tag length Distribution


De-duplication

Percentage uniques: 88.67

  All Di-Tags Unique Di-Tags
Read Pairs 16945089 15025230
Cis-close (< 10Kbp) 1079889 955693
Cis-far (> 10Kbp) 12032511 10706259
Trans 3832689 3363278