Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/ADS485.ADS485.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 152333771 152333771
Not Truncated 117626287 118332309
Truncated 34707484 34001462
Too short to map 3223590 3239758
Average length of truncated sequence 52.20 51.95
  Read 1 Read 2
Unique Alignments 123276329 117796230
Multiple Alignments 20302304 21828305
Failed To Align 5531548 9469478
Paired 100686388 100686388

Filtering

  Di-Tag Count
Valid Pairs 85602107
Invalid Pairs 15084281
Same Circularised 563280
Same Fragment Dangling Ends 1674946
Same Fragment Internal 4040254
Re-ligation 2507040
Contiguous Sequence 140272
Wrong Size 6158489
Total Pairs 100686388

Di-tag length Distribution


De-duplication

Percentage uniques: 72.38

  All Di-Tags Unique Di-Tags
Read Pairs 85602107 61958269
Cis-close (< 10Kbp) 5577436 4009001
Cis-far (> 10Kbp) 61560092 44950053
Trans 18464579 12999215