Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/ADS484.ADS484.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 153945748 153945748
Not Truncated 118428427 118832127
Truncated 35517321 35113621
Too short to map 3074492 3163854
Average length of truncated sequence 52.99 52.62
  Read 1 Read 2
Unique Alignments 126347423 120187459
Multiple Alignments 19644479 21276610
Failed To Align 4879354 9317825
Paired 103365063 103365063

Filtering

  Di-Tag Count
Valid Pairs 90417241
Invalid Pairs 12947822
Same Circularised 570739
Same Fragment Dangling Ends 1136963
Same Fragment Internal 3679894
Re-ligation 2219428
Contiguous Sequence 140198
Wrong Size 5200600
Total Pairs 103365063

Di-tag length Distribution


De-duplication

Percentage uniques: 78.77

  All Di-Tags Unique Di-Tags
Read Pairs 90417241 71226050
Cis-close (< 10Kbp) 5771784 4511682
Cis-far (> 10Kbp) 67145577 53198754
Trans 17499880 13515614