Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/ADS479.ADS479.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 149134354 149134354
Not Truncated 117183942 118049267
Truncated 31950412 31085087
Too short to map 2791531 2787771
Average length of truncated sequence 52.91 52.64
  Read 1 Read 2
Unique Alignments 121304084 113415389
Multiple Alignments 19043010 20968594
Failed To Align 5995729 11962600
Paired 97461618 97461618

Filtering

  Di-Tag Count
Valid Pairs 82944363
Invalid Pairs 14517255
Same Circularised 537620
Same Fragment Dangling Ends 1325457
Same Fragment Internal 4235388
Re-ligation 2272620
Contiguous Sequence 136015
Wrong Size 6010155
Total Pairs 97461618

Di-tag length Distribution


De-duplication

Percentage uniques: 80.83

  All Di-Tags Unique Di-Tags
Read Pairs 82944363 67047326
Cis-close (< 10Kbp) 5467977 4394341
Cis-far (> 10Kbp) 61520338 50005958
Trans 15956048 12647027