Processing Report

/research/groups/cadgen/PROJECTS/CAD_Enhancer/PChiC-HAEC-3/ADS478.ADS478.R2

ExZyVRCjrs_10-24-17_15-01-2020


Truncating and Mapping

  Read 1 Read 2
Total Reads 96882942 96882942
Not Truncated 78706234 79091938
Truncated 18176708 17791004
Too short to map 1624058 1636113
Average length of truncated sequence 52.79 52.48
  Read 1 Read 2
Unique Alignments 77342084 73215550
Multiple Alignments 12615996 13647422
Failed To Align 5300804 8383857
Paired 62236208 62236208

Filtering

  Di-Tag Count
Valid Pairs 50019687
Invalid Pairs 12216521
Same Circularised 327990
Same Fragment Dangling Ends 1114517
Same Fragment Internal 3251300
Re-ligation 2029544
Contiguous Sequence 109554
Wrong Size 5383616
Total Pairs 62236208

Di-tag length Distribution


De-duplication

Percentage uniques: 75.99

  All Di-Tags Unique Di-Tags
Read Pairs 50019687 38012344
Cis-close (< 10Kbp) 3182514 2407318
Cis-far (> 10Kbp) 35485898 27169456
Trans 11351275 8435570